生物化学

PARTⅠ:STRUCTUREANDFUNCTIONOFBIOMACROMOLECULE

Chapter1StructureandFunctionofProtein

SECTION1THECOMPOSITIONOFPROTEIN

1.1Aminoacid

1.2Peptide

1.3Somesmallpolypeptideshavebiologicalactivity

SECTION2MOLECULARSTRUCTUREOFPROTEINS

2.1Primarystructure

2.2Secondarystructure

2.3Tertiarystructure

2.4Majornoncovalentbond

2.5Quaternarystructure

SECTION3RELATIONBETWEENSTRUCTUREANDFUNCTIONOFPROTEINS

3.1Primarystructureandpotentialfunctionofprotein

3.2Spatialstructureshowsthefunctionofprotein

SECTION4CHEMICALANDPHYSICALPROPERTIESOFPROTEINS

4.1Ultravioletabsorptionspectrumofprotein

4.2Isoelectricpoint(pI)

4.3Denaturationofprotein

SECTION5ISOLATION,PURIFICATIONANDSEQUENCINGOFPROTEINS

5.1Dialysisandultrafiltration

5.2Precipitationwithacetone,saltingprecipitationandimmunoprecipitation

5.3Chromatographicseparations

5.4Electrophoresis

5.5Ultracentrifugation

SUMMARY

Chapter2ChemistryofNucleicAcids

SECTION1MOLECULARCOMPOSITIONOFNUCLEICACIDS

1.1Basiccomponents

1.2Nucleosides

1.3Mononucleotides

SECTION2MOLECULARSTRUCTURESOFDNA

2.1Primarystructureofnucleicacid

2.2Chargaffsrules

2.3SecondarystructureofDNA

SECTION3PHYSICO-CHEMICALPROPERTIESOFNUCLEICACIDS

3.1Viscosity

3.2Ultravioletabsorption

3.3DenaturationandrenaturationofDNA

3.4Hydrolysisofacidoralkali

SECTION4STRUCTURESOFRNA

4.1MessengerRNA

4.2TransferRNA

4.3RibosomalRNA

SUMMARY

Chapter3Enzymes

SECTION1THEMOLECULARCOMPOSITIONANDFUNCTIONOFENZYMES

1.1Themolecularcompositionofenzymes

1.2Theactivecenterofenzymes

1.3Manyenzymesrequirecofactors

SECTION2PROPERTIESANDMECHANISMOFENZYMECATALYSIS

2.1Remarkablepropertiesofenzymes

2.2Mechanismofenzymecatalysis

SECTION3THEKINETICSOFENZYMECATALYSIS

3.1Catalysisoccursattheactivesite

3.2Multiplefactorsaffecttheratesofenzyme-catalyzedreactions

3.3Initialrateisproportionatetoenzymeconcentration

3.4Substrateconcentrationaffectsreactionrate

3.5TheMichaelis-Mentenequationsmodeltheeffectsofsubstrateconcentration

3.6Kineticanalysisdistinguishescompetitivefromnoncompetitiveinhibition

SECTION4ZYMOGENSANDISOZYMES

4.1Zymogensandzymogenactivation

4.2Isozymesarephysicallydistinctformsofthesamecatalyticactivity

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　　本书是双语教材，总结多年教学中沉淀的经验，按照医学各专业的特点，将生物化学划分为生物大分子的结构与功能、物质代谢、基因表达和专题篇4个部分，努力构建系统化课程，从学生兴趣、认知规律和探究方便出发合理设计教材的结构，注意联系实际，适度扩大学生的知识面和应用能力，突出教材中知识结构的科学化。并以鲜活灵动的色彩、图文并茂的版面、熟悉的例子吸引刚入门的医学生，让学生感受课程学习的趣味性和挑战性，将是一系统性强、可读性强、深受学生喜爱的教材。