现代组织化学技术和方法

Chapter 1 Introduction

Section 1 The contents and theories in Histochemistry

Section 2 The basic requirements of Histochemistry methods

Chapter 2 The Tissue Preparation

Section 1 Tissue collection

Section 2 Fixation

Section 3 Tissue washing-up， dehydration and clearance

Section 4 Embedding

Section 5 Sectioning

Section 6 Adherence and mounting

Section 7 Buffer

Chapter 3 The Carbohydrate and its Derivatives Histochemistry

Section 1 Classification

Section 2 Histochemistry methods

Chapter 4 The Nucleic Acid Histochemistry

Section 1 DNA Histochemical demonstration

Section 2 Comparison demonstration of DNA and RNA Histochemistry

Chapter 5 The Lipid Histochemistry

Section 1 Overview

Section 2 Demonstration of lipids with physical methods

Section 3 Demonstration of lipids with chemical methods

Chapter 6 Enzyme Histochemistry

Section 1 Enzyme and its basic histochemical theory

Section 2 Histochemistry for common enzymes

Chapter 7 The Basic Theory of Immunohistochemistry

Section 1 Basic Immunology

Section 2 Common markers and their detection

Section 3 Basic conditions

Chapter 8 Common Methods Used in Immunohistochemistry

Section 1 Principles

Section 2 Immunofluorescence method

Section 3 Immunoenzyme technique

Section 4 Avidin-biotin method

Section 5 Protein A method

Section 6 Immunogold method and immuno gold-silver method

Chapter 9 Specificity and Sensitivity of Immunohistochemistry

Section 1 Specificity and specific staining

Section 2 Control experiment

Section 3 The methods to enhance immunohistochemical sensitivity

Chapter 10 The Double-Staining in Immunohistochemistry

Section 1 Double-staining Immunohistochemistry on serial section

Section 2 Immunofluorescence double labeling technique

Section 3 Immunoenzyme double staining technique

Section 4 Immunoenzyme-Immunofluorescence double staining

Section 5 Immunoenzyme-Immunogold double staining

Chapter 11 The Lectin Histochemistry

Section 1 Characteristics and application of lectin

Section 2 Application of lectin Histochemistry

Chapter 12 The Progress of in Situ Display

Section 1 The envision method

Section 2 The catalyzed signal amplification method

Section 3 The ferric oxide alternative method for HRP

Chapter 13 In Situ Hybridization Immunohistochemistry

Section 1 Basic principle

Section 2 Probe preparation

Section 3 Procedure of in situ hybridization histochemistry

Section 4 Factors affecting in situ hybridization

Section 5 Control test

Chapter 14 In Situ Polymerase Chain Reaction Technique

Section 1 Basic principle

Section 2 Basic types

Section 3 Procedure

Section 4 Application of in situ PeR technology

Chapter 15 Electron Microscopic Cytochemical Technique

Section 1 Electron microscopic enzyme cytochemistry technology

Section 2 Electron microscopic Immunocytochemical technique

Chapter 16 The Quantitative Assay of Histochemistry Results

Section 1 The photomicrography

Section 2 The image analyzer

Section 3 The flow cytometry

Section 4 The laser scanning confocal microscopy

References

Appendix I Preparation of Commonly Used Buffer

Appendix II Histochemistry and Immunohistochemistry Experiments

Appendix III Figures and pictures

《现代组织化学技术和方法/研究生创新教育系列教材》的主要内容包括组织标本的前期制备技术和方法，组织化学基本概念和理论以及糖、核酸、脂和酶的染色方法，免疫组织化学基本概念和理论以及染色方法，组织化学与免疫组织化学染色技术与凝集素染色技术、PCR、原位杂交和电镜技术的结合。在《现代组织化学技术和方法/研究生创新教育系列教材》最后，着重介绍了目前比较先进的定量检测仪器的基本理论和使用方法，以及与本领域有关的最近研究进展。