现代光谱学：生物物理学与生物化学例析

1Introduction

1.1Overview

1.2TheBeer-LambertLaw

1.3RegionsoftheElectromagneticSpectrum

1.4AbsorptionSpectraofProteinsandNucleicAcids

1.5AbsorptionSpectraofMixtures

1.6ThePhotoelectricEffect

1.7TechniquesforMeasuringAbsorbance

1.8Pump-ProbeandPhoton-EchoExperiments

1.9LinearandCircularDichroism

1.10DistortionsofAbsorptionSpectrabyLightScatteringorNonuniformDistributionsoftheAbsorbingMolecules

1.11Fluorescence

1.12IRandRamanSpectroscopy

1.13Lasers

1.14Nomenclature

2BasicConceptsofQuantumMechanics

2.1Wavefunctions,Operators,andExpectationValues

2.1.1Wavefunctions

2.1.2OperatorsandExpectationValues

2.2TheTime-Dependent

andTime-IndependentSchr6dingerEquations

2.2.1SuperpositionStates

2.3SpatialWavefunctions

2.3.1AFreeParticle

2.3.2AParticleinaBox

2.3.3TheHarmonicOscillator

2.3.4AtomicOrbitals

2.3.5MolecularOrbitals

2.3.6ApproximateWavefunctionsforLargeSystems

2.4SpinWavefunctionsandSingletandTripletStates

2.5TransitionsBetweenStates:Time-DependentPerturbationTheory

2.6LifetimesofStatesandtheUncertaintyPrincipleLight

3.1ElectromagneticFields

3.1.1ElectrostaticForcesandFields

3.1.2ElectrostaticPotentials

3.1.3ElectromagneticRadiation

3.1.4EnergyDensityandIrradiance

3.1.5TheComplexElectricSusceptibilityandRefractiveIndex

3.1.6Local-FieldCorrectionFactors

3.2TheBlack-BodyRadiationLaw

3.3LinearandCircularPolarization

3.4QuantumTheoryofElectromagneticRadiation

3.5SuperpositionStatesandInterferenceEffectsinQuantumOptics

3.6DistributionofFrequenciesinShortPulsesofLightElectronicAbsorption

4.7ConfigurationInteractions

4.8CalculatingElectricTransitionDipoleswiththeGradientOperator

4.9TransitionDipolesforExcitationstoSingletandTripletStates

4.10TheBorn-OppenheimerApproximation,Franck-CondonFactors,andtheShapesofElectronicAbsorptionBands

4.11SpectroscopicHole-Burning

4.12EffectsoftheSurroundingsonMolecularTransitionEnergies

4.13TheElectronicStarkEffect

5Fluorescence

5.1TheEinsteinCoefficients

5.2TheStokesShift

5.3TheMirror-ImageLaw

5.4TheStrickler-BergEquationandOtherRelationshipsBetweenAbsorptionandFluorescence

5.5QuantumTheoryofAbsorptionandEmission

5.6FluorescenceYieldsandLifetimes

5.7FluorescentProbesandTags

5.8Photobleaching

5.9FluorescenceAnisotropy

5.10Single-MoleculeFluorescenceandHigh-ResolutionFluorescenceMicroscopy

5.11PluorescenceCorrelationSpectroscopy

5.12IntersystemCrossing,Phosphorescence,andDelayedFluorescence

6VibrationalAbsorption

6.1VibrationalNormalModesandWavefunctions

6.2VibrationalExcitation

6.3IRSpectroscopyofProteins

6.4VibrationalStarkEffectsResonanceEnergyTransfer

7.1Introduction

7.2TheF6rsterTheory

7.3ExchangeCoupling

7.4EnergyTransfertoandfromCarotenoidsinPhotosynthesisExcitonInteractions

8.1StationaryStatesofSystemswithInteractingMolecules

8.2EffectsofExcitonInteractionsontheAbsorptionSpectraofOligomers

8.3Transition-MonopoleTreatmentsofInteractionMatrixElementsandMixingwithCharge-TransferTransitions

8.4ExcitonAbsorptionBandShapesandDynamicLocalizationofExcitations

8.5ExcitonStatesinPhotosyntheticAntennaComplexes

8.6ExcimersandExciplexesCircularDichroism

9.1MagneticTransitionDipolesandn-n*Transitions

9.2TheOriginofCircularDichroism

9.3CircularDichroismofDimersandHigherOligomers

9.4CircularDichroismofProteinsandNucleicAcids

9.5

11.1First-OrderOpticalPolarization

11.2Third-OrderOpticalPolarizationandNonlinearResponseFunctions

11.3Pump-ProbeSpectroscopy

11.4PhotonEchoes

11.5TransientGratings

11.6VibrationalWavepackets

11.7WavepacketPicturesofSpectroscopicTransitions

12RamanScatteringandOtherMultiphotonProcesses

12.1TypesofLightScattering

12.2TheKramers-Heisenberg-DiracTheory

12.3TheWavepacketPictureofResonanceRamanScattering

12.4SelectionRulesforRamanScattering

12.5Surface-EnhancedRamanScattering

12.6BiophysicalApplicationsofRamanSpectroscopy

12.7CoherentRamanScattering

12.8MultiphotonAbsorption

12.9Quasielastic(Dynamic)LightScattering(PhotonCorrelationSpectroscopy)

Appendix1-Vectors

Appendix2-Matrices

Appendix3-FourierTransforms

Appendix4-FluorescencePhaseShiftandModulation

Appendix5-CGSandSIUnitsandAbbreviations

References

SubjectIndex

　　本书详细论述了光学光谱理论，并介绍了如何将这些理论运用于现代分子和细胞生物物理和生物化学。全书共分12章，内容包括：电子和振动吸收，荧光共振能量转移，激广相互作用，圆二色性，相干和失相，超快泵探针和光子回波光谱，单分子和荧光相关光谱，拉曼散射，以及多重吸收。本书可供分析化学、生物化学、生物物理学和物理化学等专业高校师生、科研人员参考。　　本书详细论述了光学光谱理论，并介绍如何将这些理论运用于现代分子和细胞生物物理和生物化学。内容包括电子和振动吸收，荧光共振能量转移，激广相互作用，圆二色性，相干和失相，超快泵探针和光子回波光谱，单分子和荧光相关光谱，拉曼散射，以及多重吸收。　　本书论述基于时间相关的量子力学，为此领域专业人士提供了足够全面和详细的资料，并例举了数量众多的理论表达式或演示实验等。　　本书可供分析化学、生物化学、生物物理学和物理化学等专业高校师生、科研人员参考。