分子生物学

编译版序

AbouttheAuthor

Preface

Acknowledgments

PARTIIntroduction

1ABriefHistory

2TheMolecularNatureofGenes

3AnIntroductiontoGeneFunction

PARTⅡMethodsinMolecularBiology

4MolecularCloningMethods

5MolecularToolsforStudyingGenesandGeneActivity

PARTⅢTranscriptioninProkaryotes

6TheMechanismofTranscriptioninProkaryotes

7Operons.FineControlofProkaryoticTranscription

8MajorShiftsinProkaryoticTranscription

9DNA-ProteinInteractionsinProkaryotes

PARTⅣTranscriptioninEukaryotes

10EukaryoticRNAPolymerasesandTheirPromoters

11GeneralTranscriptionFactorsinEukaryotes

12TranscriptionActivatorsinEukaryotes

13ChromatinStructureandItsEffectsonTranscription

PARTⅤPosttranscriptionalEvents

14MessengerRNAProcessingI.Splicing

15MessengerRNAProcessingII.CappingandPolyadenylation

16OtherRNAProcessingEvents

PARTⅥTranslation

17TheMechanismofTranslationⅠ:Initiation

18TheMechanismofTranslationⅡ:ElongationandTermination

19RibosomesandTransferRNA

PARTⅦDNAReplication,Recombination,andTransposition

20DNAReplicationI:BasicMechanismandEnzymology

21DNAReplicationII:DetailedMechanism

22HomologousRecombination

23Transposition

PARTⅧGenomes

24GenomicsandProteomics

　　本书共分8个章节，以原始研究论文为基础，通过对实验的设计、对结果的分析而逐步展开对分子生物学理论的讲述，其文字通俗流畅，叙述由浅入深，可供各大专院校作为教材使用，也可供从事相关工作的人员作为参考用书使用。　　分子生物学是生命科学发展过程中诞生的一门实验性极强的新兴学科。美国著名分子生物学家RobertF.Weaver遵循这一学科发展的特点，1999年出版了MolecularBiology一书。全书以原始研究论文为基础，通过对实验的设计、对结果的分析而逐步展开对分子生物学理论的讲述，文字通俗流畅，叙述由浅入深。随着学科的迅速发展，几经修订再版的MolecularBiology第三版共有分子生物学方法，原核生物、真核生物转录，转录后加工，翻译，DNA复制、重组和转座和基因组学等八部分二十四章，书后还写有术语表。每一章节都以提出科学问题、展开研究过程开始，以提供思考习题、推荐阅读文献结束，理论讲述逻辑严密，实验过程提炼清晰，特色鲜明、内容详尽，图文并茂易读易记。是研究生和生命科学相关专业的科研、教学人员不可多得的一本优秀参考书。【作者简介】　　RobWeaverwasborninTopeka,Kansas,andgrewupinArlington,Virginia.HereceivedhisbachelorsdegreeinchemistryfromtheCollegeofWoosterinWooster,Ohio,in1964.HeearnedhisPh.D.inbiochemistryatDukeUniversityin1969,thenspenttwoyearsdoingpostdoctoralresearchattheUniversityofCalifornia,SanFrancisco,wherehestudiedthestructureofeukaryoticRNApolymeraseswithWilliamJ.Rutter.HejoinedthefacultyoftheUniversityofKansasasanassistantprofessorofbiochemistryin1971,waspromotedtoassociateprofessor,andthentofullprofessorin1981.In1984,hebecamechairoftheDepartmentofBiochemistry,andservedinthatcapacityuntilhewasnamedAssociateDeanoftheCollegeofLiberalArtsandSciencesin1995.Prof.WeaveristhedivisionaldeanforthescienceandmathematicdepartmentswithintheCollege,whichincludessupervising14differentdepartmentsandprograms.Asaprofessorofmolecularbiosciences,heteachescoursesinintroductorymolecularbiologyandthemolecularbiologyofcancer.Hedirectsaresearchlaboratoryinwhichundergraduatesandgraduatestudentsparticipateinresearchonthemolecularbiologyofabaculovirusthatinfectscaterpillars.Prof.WeaveristheauthorofmanyscientificpapersresultingfromresearchfundedbytheNationalInstitutesofHealth,theNationalScienceFoundation,andtheAmericanCancerSociety.HehasalsocoauthoredtwogeneticstextbooksandhaswrittentwoarticlesonmolecularbiologyintheNationalGeographicMagazine.HehasspenttwoyearsperformingresearchinEuropeanLaboratoriesasanAmericanCancerSocietyResearchScholar,oneyearinZurich,Switzerland,andoneyearinOxford,England.