植物病害分子流行学导论

PART Ⅰ INTRoDUCTION

CHAPTER 1 INTRoDUCl’ION TO MoLECULAR EPIDEMIOLoGY

1—1 Basic concept of molecular epidemiology

1—2 Why molecular epidemiology

1—3 Comparisons between classical and molecular methods in epidemiology

1—4 Working approaches of molecular epidemiology

1—5 About the book

References and suggested readings

PART Ⅱ MoLECULAR TECHNIQUES USED IN EPIDEMIOLOGY

CHAPTER 2 MOLECULAR MARKERS

2—1 Protein—based marker：Allozymes

2—2 DNA band—based markers

2—3 DNA sequence

References and suggested readings

CltAPTER 3 PCR—BASED TECHNIQUES

3—1 Classical PCR

3—2 Allele—specific PCR

3—3 Nested PCR

3—4 Multiplex PCR

References and suggested readings

CHAPTER 4 QUANTITATIVE REAL‘TIME PCR

4—1 Introduction

4—2 Basic real—time PCR methods

4—3 Primer and probe design for real—time PCR

4—4 Result analysis

4—5 Applications of real—time PCR

References and suggested readings

CHAmR 5 PRIMER I）ESIGN

5—1 Some basic concepts for primer design

5—2 Computer programs used for primer design

5—3 An example of primer design using a computer program

5—4 Degenerated PCR primers

PART Ⅲ MoLECULAR EPIDEMIOLOGY oF PLANT DISEASES

CHAPTER 6 PATHoGEN IDENTIFICATIoN AND DISEASE DIAGNoSIS

6—1 Introduction

6—2 Use of species—specific genes or DNA regions for developing PCR primers

6—3 Use of anonymous unique DNA regions for developing PCR primers

6—4 PCR inhibitors

Summary

References and suggested readings

CHAPTER 7 FUNGAL PATHoGEN REPRoDUCTIVE MoDES

7—1 Frequencies of genotypes

7—2 Deviation from Hardy—Weinberg expectations

7—3 Parsimony Tree Length Permutation（PTLPT）

7—4 Index of association（IA）

References and suggested readings

CHAPTER 8 DETEMINATIoN AND QUANTIFICATION OF INITIAL INOCULUM OF EPIDEMICS

8—1 Introduction

8—2 Molecular methods for quantification of initial inoculum

8—3 Examples of quantification of initial inoculum using molecular methods

Remarkable conclusion

References and suggested readings

CHPTER 9 TEMpORAL AND SPATIAL DEVELOPMENT oF PATHoGEN POPULATIoNS

9—1 Introduction

9—2 Molecular approaches

9—3 Monitoring pathogen population structures

9—4 Studying spatial—temporal dynamics of pathogen populations

9—5 Providing information needed for disease management

Summary

References and suggested readings

CHAPTER 10 PATHoGEN LONG—DISTANCE DIsPERSAL AND MIGRATION

10—1 Introduction

10—2 Pathogen lon9—distance dispersal and disease epidemics

10—3 Traditional methods to determine or infer pathogen lon9—distance dispersal

10—4 Molecular methods to study pathogen lon9—distance dispersal

10—5 Examples

Summary

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PART IV METHODOLOGY OF DATA ANLYSIS

INDEX

《植物病害分子流行学导论（英文版）》主要内容包括：Protein—based marker：Allozymes、DNA band—based markers、DNA sequence、References and suggested readings、CltAPTER 3 PCR—BASED TECHNIQUES、Classical PCR、Allele—specific PCR、Nested PCR、Multiplex PCR等等。