多肽药 : 理学、生物学和化学

Preface

The Eleventh Chinese Peptide Symposium (CPS-2010)

Acknowledgments

Cathay Award

Xiaoyu Hu Memorial Award

Chinese Peptide Symposium

Abbreviations

Session 1 Design of Peptides and Mimetics 1

Biomimetic Nanostructures Based on Enforced Folding and Assembly 3

Bing Gong

Agonist vs. Antagonist Behavior of Opioid Peptides Containing Novel

Tyrosine or Phenylalanine Analogues in Place of Tyr1 13

Grazyna Weltrowska, Irena Berezowska, Brian C. Wilkes, Carole Lemieux,

Nga N. Chung, Jinguo Ding, Yixin Lu, Peter W. Schiller,

Novel Non-Peptide Ghrelin Receptor Ligands Based on

3,4,5-Trisubstituted 1,2,4-Triazoles 20

Jean-Alain Fehrentz, Anne-Laure Blayo, Mathieu Bibian, Aline Moulin, Luc

Demange, Damien Boeglin, Céline M’Kadmi, Jean-Claude Galleyrand,

Didier Gagne, Vittorio Locatelli, Antonello Torsello, Daniel Perrissoud,

Jean Martinez,

Synthesis and Structure-Activity Relationship of Marinostatin,

An Ester-Linked Serine Protease Inhibitor 25

Misako Taichi, Toshimasa Yamazaki, Tadashi Teshima, Yuji Nishiuchi

Mitocryptides: A Novel Family of Neutrophil-Activating Peptides Derived

from Mitochondrial Proteins and Their Regulatory Mechanisms 30

Hidehito Mukai, Tetsuo Seki, Akiyoshi Fukamizu, Yoshiaki Kiso

Novel Opioid Peptide Derived Antagonists Containing 3-(2,6- Dimethyl-4-

Carbamoylphenyl)-Propanoic Acid (Dcp) in Place of Tyr1 33

Peter W. Schiller, Grazyna Weltrowska, Thi M.-D. Nguyen, Carole Lemieux,

Nga N. Chung, Tze Keong Lum, Yoon Wui Leow Augustine, Yixin Lu

Design, Synthesis and Evaluation of Antitumor Activity against

Human Cancer Cell Lines, Sansalvamide Analogues 39

Xiaoli Zhen, Yihua Yang, Junzhang Li, Xia Tian, Hua Zhang, Shouxin Liu

Session 2 Peptide Synthesis and Preparation 41

Regioselective Disulfide Bond Formation during SPS of Human Relaxin-3:

Evaluation of An Alternative Method for Removal of the S-Acm Groups via

Reaction with Silver Triflate and Subsequent Mild Aqueous

DMSO Oxidation 43

Feng Lin, John D. Wade

Synthetic Studies of Membrane Proteins: Subunit C of Human F1Fo-ATP

Synthase and Voltage Sensor Only Protein (78-222) 47

Saburo Aimoto, Ken’ichiro Nakamura, Takeshi Sato, Toru Kawakami,

Toshiaki Hara

One-Pot Synthesis of Rigid Cyclic N-Methyl-γ-Peptides 54

Zhongzhu Chen, Liangchun Li, Menglan Lv, Jin Zhu and Jingen Deng

Tbfmoc Methioine: Its Synthesis and Application for the Solid Phase

Synthesis of Ubiquitin Analogues and Product Purification 58

Naifeng Hu, Zhi Jin, Pu Wang

Intein-Mediated Biosynthesis of Bracelet Cyclotide 63

Xinya Hemu, Lin Cai, Clarence T. T. Wong, James P. Tam

Convergent and Atom-Economic Synthesis of Phosphinopeptides 67

Fanhua Meng, Jiaxi Xu

One-Step Oxidative Folding of A Novel Cystine-Knot Cyclotide

Under Denaturation Conditions 69

Clarence T. T. Wong, Misako Taichi, Hideki Nishio, Yuji Nishiuchi,

James P. Tam

Synthesis of Angiotensin I-Converting Enzyme Inhibitory Peptides and

Their Self-Assembling Microstructure Characterization 72

Dawei Li, Yan Ding, Gang Cao, Ruijie Zhang, Jin Ren, Chuanguang Qin

Fast Automated Synthesis of An Antimicrobial Peptide Analog

Using UV-Monitoring on the Tribute? 77

German E. Fuentes, Christina A. Chantell, Hirendra Patel, Mahendra

Menakuru

Synthesis of Cyclic Peptides by Alknye-Azide Click Chemistry Cyclization 81

Liangyou Wang, Dongdong Lu, Mingxing Lu

Thioester Preparation through O-S Acyl Shift under Acidic Condition 83

Khee Dong Eom, Young Soon Shin, James P. Tam

A Novel Strategy for Total Solid-Phase Synthesis of

Kahalalide A Derivatives 86

Tingting Wei, Jin Ren, Xiaoya Shang, Gang Cao, Chuanguang Qin

Session 3 Methodologies in Peptide Research 91

Protein Synthesis Using Novel Ligation Partners 93

Wen Hou, Renliang Yang, Fupeng Li, Xiaohong Zhang, Chuanfa Liu

Expanding Role of Mass Spectrometry for Characterization of Proteins 97

Pui-kin So, Zhongping Yao

Effect of pH on the Self-Assembly of Surfactant-Like Peptides 102

Shuyi Han, Jing Wang, Hai Xu

Conformational Isomers in N-Methylzygosporamide Cyclic Pentapeptides 110

Yihua Yang, Shirui Gao, Xianrui Yuan, Shouxin Liu, Wei Meng, Guodi Qi

Interaction between Amyloid-β-Peptide (1-40) and Its Aggregation

Inhibitors Studied by Circular Dichroism 112

Qing Zeng

Disulfide Bond Formation Directed by Watson-Crick Base Pairing 116

Fupeng Li, Xiaohong Zhang, Wen Hou, Chuanfa Liu

Interfacial Adsorption of Novel Lipopeptide Surfactant C14K3: A Combined

Study of Spectroscopic Ellipsometry and Neutron Reflection 120

Donghui Jia, Kai Tao, Jiqian Wang, Xiubo Zhao, Hai Xu, John R. P.

Webster, Jian R. Lu

Session 4 Peptide Therapeutics and Application 135

Peptide-Based Nanoparticles for Cancer Therapy and Imaging 137

Yuanpei Li, Kai Xiao, Juntao Luo, Wenwu Xiao, Joyce Lee,

Lorenzo Berti, Kit S. Lam

Involvement of Na+,K+-ATPase in Leukemia Cell Apoptosis

by Modulating Sensitivity 145

Wu Yin, Wei Cheng, Wei Chen, Dan Cao, Su Feng, Xinyin Chen, Jin Zhao,

Yilin Shi, Zichun Hua

Short Constrained Dipeptide Mimic Oligomers as Uncharged Cell

Penetrating Compounds 157

Lubomir L. Vezenkov, Christophe André, David Paramelle, Marie Maynadier,

Jean-Francois Hernandez, Marcel Garcia, Monique Calmès, Gilles Subra,

Ettore Benedetti, Jean Martinez, Muriel Amblard

Synthesis and Biological Properties of Peptide-siRNA Conjugates 161

Zhenjun Yang, Xiaofeng Wang, Yang Liu, Yue Chen, Feilin Nie, Lihe Zhang

FGF-P Facilitates Cell Proliferation Following Ionizing Radiation 167

Yeping Tian, Shanmin Yang, Mei Zhang, Yongbing Cao, Bingrong Zhang,

Lei Zhang, Liangjie Yin, Kunzhong Zhang, Steven G. Stwarts, Bruce M.

Fentona, Paul Okunieff, Lurong Zhang

Synthesis, Evaluation and Molecular Modeling Study of L-2-amino-7/8-

Bromoalkanoic Acid Derivatives as Histone Deacetylase Inhibitors 172

Dawei Huang, Xiaohui Li , Zhilong Xiu, Norikazu Nishino

Thirteen-Membered Cyclic Tetrapeptides as Histone Deacetylase Inhibitors 177

Hongfang Chi, Yasuhiko Otsuka, Tamaki Kato, Norikazu Nishino,

Akihiro Ito, Minoru Yoshida

Antimicrobial Peptide May be Desirable Candidate for the Treatment of

Multidrug-Resistant Cancer & Bacteria: A Simple Introduction to the

Therapeutic Effects of A Novel Antimicrobial Peptide Polybia-MPI 182

Kairong Wang, Bangzhi Zhang, Jiexi Yan, Wei Zhang, Ru Chen,

Jingjing Song, Rui Wang

Remodeling of Cyclic Tetrapeptides Framework of Chlamydocin as

Histone Deacetylase Inhibitors 188

Jiani Li, Xiaohui Li, Yasuhiko Otsuka, Tamaki Kato, Norikazu Nishino,

Akihiro Ito, Minoru Yoshida

Identification of Novel HLA-A*0201-Restricted Cytotoxic T

lymphocyte Epitopes from PLAC1 in Breast Cancer 192

Wei Liu, Mingxia Zhai, Chao Dai, Yuanming Qi, Yanfeng Gao

Session 5 Bioactive Peptides and Pharmacology 197

In Vitro Characterization for the Effects of Insect Kinins on Mouse

Distal Colonic Motility 199

Longfei Yang, Yang Xiao, Yun Huang, Guoning Ma, Zhiyuan Cong,

Shouliang Dong

Evaluation of the Bioequivalence of the 20 mg /10 ml Doses of Two

Injectable Preparations of Eptifibatide: A Randomized, Open-Label,

Two-Period Crossover Study in Healthy Adult Male Chinese Volunteers 204

Yanhui Gao, Baoqiu Li, Jun Sun

The Characteristics of Sleep-Wake States and Potential Neuronal

Targets for Neuropeptide S 210

Peng Zhao, Yufeng Shao, Min Zhang, Kun Fan, Rui Wang, Yiping Hou

Antibacterial Activity of Surfactant-Like Linear Amphipathic

Cationic Peptides 218

Jing Hu, Cui X. Chen, Sheng Z. Zhang, Hai Xu, Jian R. Lu

Comparison of the Pharmacological Functions of the NPFF1

and NPFF2 Receptors Ligands 229

Quan Fang, Ning Li, Rui Wang

Preparation of Monomeric B27 Lys Destripeptide Insulin by

Intein Mediated Expression in E. Coli 236

Lujuan Li, Helong Hao, Yuanhao Qiao, Ting Chen

Endomorphins Prevent Cardiac Oxidative Damage Induced

by Cadmium Chloride in Rats 241

Pin Gong, Fuxin Chen, Rui Wang

Study on Anticancer Mechanism of Cyclic Tetrapeptide HDACIs 246

Shimiao Wang, Xiaohui Li, Zhilong Xiu, Norikazu Nishino 246

Biological Roles of Hemokinin-1: A Novel Member of

the Tachykinin Family 251

Ziqing Kong, Wenle Yang, Caiyun Fu, Youli Zhao, Yan Tao, Rui Wang

Author Index 257

Subject Index 260

The 11th Chinese International Peptide Symposium (CPS-2010), hosted by Lanzhou University, was held in Lanzhou, China, July 5-8, 2010. More than 300 participants, from 12 countries and districts as well as represents from 30 or so related companies attended this symposium. Thus, it was gratifying to see that this meeting retained both its international flavor and participants loyalty at a time when there are a great many symposia held each year on similar subjects. The goal of this symposium was to provide a forum for exchange of ideas, review of the progress in the field of peptide research, encourage the cooperation between the international and Chinese scientific communities. We believe that this goal had been achieved.

　　The scientific program, thanks to the insights and efforts of the program committee, was extraordinary rich, diverse and exciting. It was comprised of 14 sessions with 22 plenary lectures, 37 invited lectures and 116 poster presentations, concerning bioactive peptides, peptide immunology, structure and conformation of peptides and proteins, molecular diversity and peptide libraries, synthetic methods, de novo design and synthesis of proteins and peptides, ligand-receptor interactions, the chemistry-biology-interface, self-assembling peptides and challenging problems in peptides.

　　Dr. John D. Wade and Dr. Yanmei Li were the recipients of ‘Cathay Award’ sponsored by H. H. Liu Education Foundation, offering their seminal contributions to peptide science and the Chinese International Peptide Symposium. Dr. Saburo Aimoto and Dr. Luhua Lai were the recipients of ‘Xiaoyu Hu Memorial Award’ sponsored by Hainan Zhonghe (Group) Co. Ltd, offering their great contributions in peptide science, peptide application and Chinese International Peptide Symposium. Especially, it should be mentioned that ‘Xiaoyu Hu Memorial Award’ was first set up at the 11th CPS to commemorate Professor Xiaoyu Hu for his great contribution to the development of peptide science and peptide application in China. Four young outstanding scientists were selected by the International Program Committee to receive the Young Peptide Scientist Award , which was sponsored by Hainan Zhonghe (Group) Co. Ltd.

　　The enthusiastic cooperation and prominent contributions were gratifying and the active response of the invited speakers contributed to the success of the symposium. The presentations were of excellent caliber and represented the most current and significant aspects of peptide science.

　　I would like to acknowledge academicians of chinese academy of sciences, Professor Youshang Zhang and Professor Yundong Wu for their kindness in supporting and serving as the chairman of the awarding committee for the ‘Cathay Award’ and ‘Xiaoyu Hu Memorial Award’ respectively. The success of the 11th CPS was largely due to the dedication, enthusiasm and hard work of the local organizing committee, who for the most part consisted of volunteers. As Symposium Chair, I am appreciated for their contributions very much.