机能学综合实验指导

CHAPTER ONE INTRODUCTION

Section 1 The Content， Purpose and Requirements of Functional Experiments Course

1.1.1 Content

1.1.2 Purpose

1.1.3 Requirements

1.1.4 Format of Experiment Report

Section 2 Basic Knowledge Required in the Functional Experiments

1.2.1 Basic Knowledge about Laboratory Animals

1.2.2 How to Calculate the Drug Dosage to Animals

1.2.3 Animal Handling and Different Routes of Administration

1.2.4 Anesthesia of Laboratory Animals

Section 3 Common Apparatus Used in Functional Experiments

1.3.1 RM6240C Channel Physiological Signal Acquiring and Processing System

1.3.2 BL-420 Data Acquisition and Analysis System for Life Science

1.3.3 BI-2000 System of Image Processing and Analysis

1.3.4 Computer and Local Network Technique Used in the Teaching

CHAPTER TWO BASIC EXPERIMENTS IN FUNCTIONAL EXPERIMENTS

Section 1 Analog Experiments on the Computer or Machine

2.1.1 Analog Experiments of Electro-physiology of the Calamarys Huge Axon on the Computer

2.1.2 Analog Experiments of Mechanics of Muscle Contraction on the Computer

2.1.3 Analog Experiments of One Compartment Open Model on the Machine

2.1.4 Analog Experiments of Two Compartment Open Model on the Machine

Section 2 Functional Experiments on Isolated Preparations

2.2.1 Preparation of Sciatic Nerve-Gastrocnemius （SNG） Samples

2.2.2 Influences of the Muscle Contraction through Different Stimulation Intensities

2.2.3 Influences of the Muscle Contraction through Different Stimulation Frequencies

2.2.4 Preparation of Isolated Toad Heart

2.2.5 Observation and Recording of Toads Heart Beat ..

2.2.6 The Effects of Electrolyte on the Heart Activity

2.2.7 The Physiological Characters of the Rabbit Small Intestine Segment in-vitro

2.2.8 The Influence of Drugs on Isolated Toad Heart

2.2.9 Effect of Drugs on Receptors in Isolated Intestine Smooth Muscle of a Rabbit

Section 3 Functional Experiments on Intact Preparations

2.3.1 The Discharge of Rabbit Depressor Nerve

2.3.2 Factors that Affect the Urine Formation in a Rabbit

2.3.3 Comparing the Effects of Pentobarbital Sodium on Mice by Different Routes of Administration

2.3.4 The Surface Anesthesia Effect of Tetracaine and Procaine on Rabbit Eyes

2.3.5 The Epidural Anesthesia Effect of Procaine on Rabbits

2.3.6 Mensuration Dose-effect Curve of NA on Blood Pressure in Rabbits

2.3.7 Effect of Chlorpromazine on Body Temperature Regulation in Rats

CHAPTER THREE INTEGRATED AND ADVANCED EXPERIMENTS INFUNCTIONAL EXPERIMENTS

Section 1 Multi-physiological Index Observation on Animal Body

3.1.1 Nervous and Humoral Regulation of Arterial Blood Pressure in a Rabbit

3.1.2 The Stimulus Effects on the Motor Area in Pallium of a Rabbit

3.1.3 Nervous Regulation of the Respiratory Movement in a Rabbit

3.1.4 Mensuration of ED50 of Sodium Pentobarbital in Mice

3.1.5 Effects of Drugs on Cardiovascular System of a Rabbit

Section 2 Preparation of Animal Disease Model

3.2.1 A Rabbit Model for Hyperkalemia

3.2.2 An Analysis of Experimental Edema in Toads

3.2.3 Characterization of the Different Types of Hypoxia

3.2.4 A Rabbit Model of Acute Hemorrhagic Shock

3.2.5 The Effects of Ammonia on Hepatic Encephalopathy

Section 3 Therapeutic Effects of Drugs on Animal Body

3.3.1 Intoxication and Detoxication of Organophosphorus

3.3.2 Anticonvulsant Effect of Drugs in Mice

3.3.3 Observation of Analgesic Action of Drugs with Turning Trunk Method in Mice

3.3.4 Toxic Effect of Streptomycin Sulfate and Antagonistic Effect of Calcium Chloride on a Rat

3.3.5 Diuretic Effect of Drugs on a Rabbit

3.3.6 Effect of Glucocorficoids on Permeability of Capillary Vessels in Mice

CHAPTER FOUR EXAMINATION ITEMS IN FUNCTIONAL EXPERIMENTS

Section 1 Observation and Preparation of Isolated Animal Samples

4.1.1 Observation and Recording of Toads Heart Bea

4.1.2 Preparation of Sciatic Nerve-Gastrocnemius （SNG） Samples

Section 2 Analysis and Preparation of Animal Disease Model

4.2.1 A Rabbit Model of Acute Hemorrhagic Shock

4.2.2 Nervous and HumoralRegulafion of Arterial Blood Pressure in a Rabbit

4.2.3 Factors that Affect the Urine Formation

Section 3 An Analysis of Effects of Different Drugs on Animals or Samples

4.3.1 Effect of Drugs on Receptors in Isolated Intestine Smooth Muscle

4.3.2 Analysis of the Effects of Drugs Acting on the Central Nerve System

APPENDIX

Preparation and Component of Experimental Solution

Physiological Constants of Laboratory Animals

REFERENCE

《机能学综合实验指导》的第一章介绍了实验内容、目的、要求、实验动物基本知识和实验仪器，这部分内容由郭涛、赵岩同学和杨爽、倪虹老师编写。其余三章内容中涉及生理学专业的实验内容由李静老师编写，涉及病理生理学专业的实验内容由倪虹老师编写，涉及药理学专业的实验内容由张京玲老师编写，杨卓老师承担了全书的英文审校工作。