医学生物学实验

Chapter1 Introduction to the Medical Biology Laboratory

11 Objectives of a Teaching Medical Biology Laboratory

12 Laboratory Rules

13 Laboratory Safety

14 Common Laboratory Equipments

15 Experimental Methods in Medical Biology

16 Evaluation of Experimental Data and Laboratory Reports

Chapter 2 Experiments in Medical Biology

Experiment 1 The Use of Glass Pipets and Pipettors N

Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins

Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie BriHiant Blue As Tay NN

Experunent 4Quantitative Determination of Proteins by Folin-phenol Assay

Experiment 5 Determination oF Proteins by UV Absorption

Experiment 6 Separation of Arrmio Acid MixtIHes by Ion-exchange Chromatography

Experiment 7 Separation of Serum y-globulins by Ion-exchange Chromatography

Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane

Electrophoresis

Experiment 9 Separation and Purity Determination of Serum y-globulins by SDS-polyacrylamide Gel Electrophoresis

Experiment 10 Determination of Serum Glutamicpyruvic Transaminase Activity

Experiment 11 Determination of Serum Urea Nitrogen Content

Experiment 12 Determination of Blood Glucose Concentration

Experiment 13 Subculture of Adhesion Cells NN

Experiment 14 Counting and Determination of Live/Dead Cells

Experiment 15 Preparation of Cell Samples for Observation of Cell Morphologies

Experiment 16 Mitosis and Meiosis of Cells

Experiment 17 Intracellular Distribution of Biomacromolecules

Experiment 18 Isolation of Subcellular Components

Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells

Experiment 20 Banding Pattern of Human Chromosomes

Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells

Experiment 22 Isolation,Amplification and Identification of the pactin Gene Fragment from Mouse Liver Cells

Experiment 23 Purification of Target DNA and Generation of Recombinant DNA

Experiment 24 Transformation and Screening of Recombinant DNA

Experiment 25 Determination of Serum Proteins Using Western Blotting

《医学生物学实验（英文版）》由2部分组成。第一部分概括地介绍医学生物学实验的目的、实验室规范、实验安全、常用实验室仪器、实验方法以及实验数据的评价。第二部分包括25个实验，包括生物分子的分析、细胞和超微结构的分析、分子生物学实验、医学遗传学实验、实验技术在医学研究中的应用等。书中所介绍的所有实验都是经过验证的，而且学生在正常实验课期间均可完成。