分子生物学实验指导

Experiment 1

Preparation of Plasmid DNA

Experiment 2

Restriction Enzyme Digestion and Agarose Gel Electrophoresis

Experiment 3

Polymerase Chain Reaction（PCR）

Experiment 4

DNA Purification and Construction of Recombinant DNA

Experiment 5

Preparation of Competent E.coli Cells

Experiment 6

Transformation of Recombinant DNA

Experiment 7

Identification and Characterization of Positive Clones

Experiment 8

Isolation ofTotal RNA from Animal Tissues

Experiment 9

Molecular Hybridization

Experiment 10

Reverse Transcription—Polymerase Chain Reaction（RT-PCR） and TA-cloning

Experiment 11

Expression and Purification of a Fusion Protein from E.coli

Experiment 12

Electrophoretic Mobility Gel Shift Assay（EMSA）to Detect DNA—Protein Interactions

Appendix I

Brief Introduction to the NCBI Database

Appendix II

Vector Information

Appendix III

Instructions on How to Use the Equipment

References

《分子生物学实验指导（英文版）》以科研思路的培养为基本出发点，以真核生物基因为实验材料，涵盖DNA提取、分子克隆、蛋白质表达纯化、RNA提取、表达谱分析、转录水平调控分析方法等多个基础实验。各实验既可以单独开设，也可以将前后联系的独立实验整合为综合实验。每个实验分为背景知识与概念、操作步骤、注意事项以及思考题等内容，并在书后附仪器的使用说明、基本操作的介绍，网络资源使用简介等补充知识。全书双色印刷。