机能实验学

chapter 1. an introduction of functional experimentation

chapter 2. basic knowledge and theory

section 1. biological characteristics and application of commonly used laboratory animals

section 2. biological data acquisition systems

section 3. selection and design principles of medical scientific research topics

section 4. medical information retrieval

section 5. writing methods of scientific papers and lab reports

section 6. laboratory safety and rules

chapter 3. basic methods of animal experiments

section 1. animal capture and restraint

section 2. animal numbering

section 3. drug administration for laboratory animals

section 4. anesthesia of laboratory animals

section 5. unhairing of laboratory animals

section 6. blood collection of laboratory animals

section 7. euthanasia methods of laboratory animals

chapter 4. basic techniques in experimental animal surgical operation

section 1. traditional surgical instruments

section 2. technique of animal catheterization

section 3. haemostasis and technique of knots making

section 4. technique of wound sutures （stitches）and sutures out

chapter 5. basic experiments

experiment 1. the nature of muscle contraction

experiment 2. measurement of conduction velocity of action potential and excitability from neural trunk

experiment 3. determination of hemoglobin content and red blood cell osmotic fragihty

experiment 4. premature contraction and compensatory pause

experiment 5. chemicals on the regulation of ex vivo heart activities

experiment 6. measurement of human arterial blood pressure

experiment 7. recording of human electrocardiogram

experiment 8. pulmonary function tests

experiment 9. regulation of respiratory movements

experiment 10. physiological properties of the isolated gastrointestinal smooth muscle

experiment 11. factors that influencing urine formation

experiment 12. damage effects of unilateral labyrinth and cochlear potential induction from guinea pigs

experiment 13. examination of visual fields and scotornas

experiment 14. analysis of reflex arc and the basic features of the reflex activities

experiment 15. experimental pulmonary edema in rabbits

experiment 16. effect of different factors on hypoxic tolerance

experiment 17. influence of ph of body fluids on drug absorption

experiment 18. one-compartment open model and calculation of the pharmacokinetic parameters

experiment 19. two-compartment open model and calculation of the pharmacokinetic parameters

experiment 20. determination of medial lethal dose （lds0） of drug

experiment 21. the antergic experiments of drng-the binding assay of radio-ligand and receptor

experiment 22. intoxication and detoxication of organophosphates

experiment 23. effects of drugs by different dosage forms

experiment 24. effects of drugs by different routes of administration

experiment 25. basic effects of drugs

experiment 26. factors influencing drug effects

experiment 27. observation of analgesic action of drugs with writhing method

experiment 28. anticonvulsant effect of the drug

experiment 29. effects of drugs on learning and memory of animal （mice step-down experiment. rat shuttle box experiment. morris water maze experiment）

experiment 30. effects of hydrocortisone on mice ear swelling induced by xylol

experiment 31. anti-inflammatory effects of dexamethasone on rat paw edema

experiment 32. effects of cardiac glycosides on heart failure

experiment 33. antibacterial experiment of antibacterial agents in vitro

experiment 34. antibacterial experiment of antibacterial agents in vivo

chapter 6. comprehensive experiments （ ）

experiment 1. different types of hypoxia

experiment 2. hyperkalemia in rabbits

experiment 3. disseminated intravascular coagulation in rabbit

experiment 4. disorders of acid-base balance and its experimental therapy in rabbits

experiment 5. ischemia and reperfusion injury in intestine

experiment 6. acute endotoxic shock

experiment 7. hemorrhagic shock and alterations of microcirculation in rabbit

experiment 8. regulation of the cardiovascular system and acute cardiac failure

experiment 9. effects of experimental pneumothorax on respiration. circulation and acid-base balance in rabbits

experiment 10. acute respiratory distress syndrome and pharmacotherapy

experiment 11. hepatic encephalopathy and its treatment

experiment 12. determination of endogenous creatinine clearance rate and excretion fraction of sodium filtration in acute renal failure

experiment 13. reproduction of multiple organ dysfunction syndrome

experiment 14. the effect of drug and bioactivator on the isolated tracheal strips of cavy

experiment 15. determination of the pharmacokinetic parameters of sulfa drugs in rabbits with renal failure

chapter 7. exploratory experiments

chapter 8. case discussion and paper analysis

references

appendix

《机能实验学（第2版）（英文版）》主要包括三部分内容，一是较系统地介绍机能学实验基本知识、动物学实验基本技术、科研基本方法以及实验室安全规则等，强调了对实验基本知识和技术方法的掌握；第二部分为生理学、病理生理学和药理学的一些经典实验内容，共由34个实验项目组成，主要用于促进对医学理论知识的理解；第三部分由综合性实验、探索性实验、病例讨论、高水平论文分析等内容组成，主要用于训练和提高学生的综合实验能力和科研素质。本教材在形式上以英文为主，对章节段落的标题及少数较难的专业词汇则插人中文注释。本教材涉及大量的医药学基础与临床专业英语词汇，因此除可作为生理学、病理生理学和药理学等实验双语教材外，还可作为各专业学生学习医学专业英语的良好课外读物。